一、可以，使用两个手机号注册两个支付宝账户就可以使用两个支付宝账号了。
但是有一点要注意，由于支付宝和电话卡是实名认证的，这个身份证注册的第一个支付宝账号是母账号，之后注册的都是子账号。母账号拥有支付宝所有的功能，可以使用所有的服务，子账号在功能和服务上会受到限制。
另外，一个身份证号关联的所有支付宝账号芝麻分相同，花呗借呗等额度是这些支付宝账号额度总和，比如你的花呗额度是5000，意思是这些支付宝账号共计可以用5000花呗额度。
二、手机上使用两个支付宝的操作方法：
1、打开手机支付宝APP，进入支付宝APP主界面之后，选择右下方“我的”点击进入。
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2、进入“我的”个人中心之后，点击右上角的“设置”点击进入。
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3、进入设置界面之后，点击“换账号登陆”进入账号切换界面。
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4、进入账号切换界面之后，点击“换个新账号登陆”进入支付宝登陆界面。
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5、输入双卡双待手机的手机号即可登陆支付宝账号，
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6、登陆完成后，返回第4步两个支付宝账号就可以随意切换使用。
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