英语翻译

Question

本论文利用大肠杆菌原核表达系统，以亚洲Ⅰ型口蹄疫病毒 (Foot-and-mouth disease virus,FMDV) 为研究对象,通过PCR 扩增得到非结构蛋白3D 片段, 然后与载体 pET-30a连接成重组质粒493-pET30a (pET30a-3D),转化宿主菌 BL21，IPTG 诱导表达目的蛋白，表达产物经 SDS-PAGE 检测，证明在大肠杆菌中成功地表达了口
蹄疫非结构蛋白 3D。表达蛋白可用于 FMDV感染动物的鉴别诊断，为 3D 蛋白的生物学功能和抗原表位研究以及构建更加有效的疫苗奠定了基础。
本论文利用大肠杆菌原核表达系统，以亚洲Ⅰ型口蹄疫病毒 (Foot-and-mouth disease virus,FMDV) 为研究对象,通过PCR 扩增得到非结构蛋白3D 片段, 然后与载体 pET-30a连接成重组质粒493-pET30a (pET30a-3D),转化宿主菌 BL21，IPTG 诱导表达目的蛋白，表达产物经 SDS-PAGE 检测，证明在大肠杆菌中成功地表达了口蹄疫非结构蛋白 3D。表达蛋白可用于 FMDV感染动物的鉴别诊断，为 3D 蛋白的生物学功能和抗原表位研究以及构建更加有效的疫苗奠定了基础。

Answer 1

This paper escherichia coli original nuclear expression system in Asia, Ⅰ type of foot-and-mouth disease (Foot - and - mouth FMDV) with virus get, as the research object, by PCR nonstructural proteins obtained with 3D clips and 30a connected into carrier pET - recombinant plasmid 493 - pET30a (pET30a - 3D), transformation induced IPTG BL21, host bacterium, target protein expression by sds-page express product testing, proof in e. coli successfully expressed in mouth

Hoof epidemic unstructured protein 3D. Express protein can be used in the differential diagnosis of FMDV infected animals, for 3D protein biological function and antigen table a research and building more effective vaccine laid a foundation.

Proof in escherichia coli successfully expressed foot-and-mouth disease unstructured protein 3D. Express protein can be used in the differential diagnosis of FMDV infected animals, for 3D protein biological function and antigen table a research and building more effective vaccine laid a foundation

Answer 2

In this thesis, a prokaryotic expression system of E. coli, foot and mouth disease virus type Asia-Ⅰ (Foot-and-mouth disease virus, FMDV) was studied by PCR amplified fragment of non-structural protein 3D, and then connected with the carrier of recombinant pET-30a Plasmid 493-pET30a (pET30a-3D), into host bacteria BL21, IPTG induced protein expression products by SDS-PAGE testing to show that successfully expressed in E. coli of the mouth
Hoof epidemic of non-structural protein 3D. Protein can be used for differential diagnosis of FMDV infection in animals, for 3D protein biological functions and antigenic epitopes, and building a more effective vaccine basis.本回答被提问者采纳